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standard size marker for pfge  (New England Biolabs)


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    New England Biolabs standard size marker for pfge
    Standard Size Marker For Pfge, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/standard+size+marker+for+pfge/MidRange+PFG+Marker/pmc06810192__18___1548___Techapp___s1-55-12-17
    Average 96 stars, based on 443 article reviews
    standard size marker for pfge - by Bioz Stars, 2026-10
    96/100 stars

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    Marker:

    Article Title: Non- Leishmania Parasite in Fatal Visceral Leishmaniasis–Like Disease, Brazil
    Article Snippet: Briefly, promastigote samples were immobilized in 2% low-melting temperature agarose plugs, incubated in lysis solution (0.5M EDTA pH 9.0, 1% sodium lauroyl sarcosinate, 0.5 mg/mL proteinase K) for 48 hours at 45°C and then loaded in gels running in a contour-clamped homogeneous electric field apparatus (CHEF DR II, BioRad, USA) at 14°C, at 4.5 V cm-1 over 48 hours with a 50- to 120-s pulse ramp time. .. A Lambda Ladder of size range 50–1,000 kb was used as a standard size marker for PFGE (New England Biolabs). ..



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    FIG. 1. <t>PFGE</t> profiles of 20 A. baumannii isolates (lanes 1 to 20) used in study phase I digested with ApaI. The profiles were generated in laboratory B. Isolates represent both outbreak-related and sporadic strains. The participating laboratories were blinded to the identities of the isolates. Lanes M, <t>bacteriophage</t> <t>lambda</t> ladder; lanes R, A. baumannii COL 20820, which was the external reference standard used for normalization of the gels and which was run on every fourth to fifth lane. See Table 1 for details about the strains.
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    FIG. 1. PFGE profiles of 20 A. baumannii isolates (lanes 1 to 20) used in study phase I digested with ApaI. The profiles were generated in laboratory B. Isolates represent both outbreak-related and sporadic strains. The participating laboratories were blinded to the identities of the isolates. Lanes M, bacteriophage lambda ladder; lanes R, A. baumannii COL 20820, which was the external reference standard used for normalization of the gels and which was run on every fourth to fifth lane. See Table 1 for details about the strains.

    Journal: Journal of Clinical Microbiology

    Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

    doi: 10.1128/jcm.43.9.4328-4335.2005

    Figure Lengend Snippet: FIG. 1. PFGE profiles of 20 A. baumannii isolates (lanes 1 to 20) used in study phase I digested with ApaI. The profiles were generated in laboratory B. Isolates represent both outbreak-related and sporadic strains. The participating laboratories were blinded to the identities of the isolates. Lanes M, bacteriophage lambda ladder; lanes R, A. baumannii COL 20820, which was the external reference standard used for normalization of the gels and which was run on every fourth to fifth lane. See Table 1 for details about the strains.

    Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

    Techniques: Generated

    FIG. 2. PFGE profiles of representative A. baumannii isolates (lanes 1 to 5) digested with ApaI and obtained by using a highly standardized protocol for PFGE, as performed in laboratories A to C (panels A to C, respectively). Lanes R, A. baumannii COL 20820, which was the external reference standard. See Table 1 for details about the strains.

    Journal: Journal of Clinical Microbiology

    Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

    doi: 10.1128/jcm.43.9.4328-4335.2005

    Figure Lengend Snippet: FIG. 2. PFGE profiles of representative A. baumannii isolates (lanes 1 to 5) digested with ApaI and obtained by using a highly standardized protocol for PFGE, as performed in laboratories A to C (panels A to C, respectively). Lanes R, A. baumannii COL 20820, which was the external reference standard. See Table 1 for details about the strains.

    Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

    Techniques:

    FIG. 3. Computer-assisted analysis of PFGE patterns generated at three different laboratories in study phase I confirmed the interpreta- tions obtained at the local laboratories and showed similarity values of 87% for the corresponding isolates processed at different laboratories.

    Journal: Journal of Clinical Microbiology

    Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

    doi: 10.1128/jcm.43.9.4328-4335.2005

    Figure Lengend Snippet: FIG. 3. Computer-assisted analysis of PFGE patterns generated at three different laboratories in study phase I confirmed the interpreta- tions obtained at the local laboratories and showed similarity values of 87% for the corresponding isolates processed at different laboratories.

    Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

    Techniques: Generated

    FIG. 4. Gel images of PFGE patterns of 30 A. baumannii isolates (lanes 21 to 50), to whose identities the participating laboratories were blinded, from 10 hospital outbreaks, represented by 3 isolates each. Each laboratory (laboratories A to C; panels A to C, respectively) therefore processed 10 isolates from the 10 outbreaks; laboratory A, strains 41 to 50; laboratory B, strains 21 to 30; and laboratory C, strains 31 to 40. Lanes M, bacteriophage lambda ladder; lanes R, A. bauman- nii COL 20820, which was the reference standard and which was run on every fourth to fifth lane. See Table 2 and Fig. 5 for details about the strains.

    Journal: Journal of Clinical Microbiology

    Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

    doi: 10.1128/jcm.43.9.4328-4335.2005

    Figure Lengend Snippet: FIG. 4. Gel images of PFGE patterns of 30 A. baumannii isolates (lanes 21 to 50), to whose identities the participating laboratories were blinded, from 10 hospital outbreaks, represented by 3 isolates each. Each laboratory (laboratories A to C; panels A to C, respectively) therefore processed 10 isolates from the 10 outbreaks; laboratory A, strains 41 to 50; laboratory B, strains 21 to 30; and laboratory C, strains 31 to 40. Lanes M, bacteriophage lambda ladder; lanes R, A. bauman- nii COL 20820, which was the reference standard and which was run on every fourth to fifth lane. See Table 2 and Fig. 5 for details about the strains.

    Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

    Techniques:

    FIG. 5. Computer-assisted analysis of PFGE patterns generated at three different laboratories in study phase II correctly identified 3 isolates from each of 10 outbreaks among the isolates investigated.

    Journal: Journal of Clinical Microbiology

    Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

    doi: 10.1128/jcm.43.9.4328-4335.2005

    Figure Lengend Snippet: FIG. 5. Computer-assisted analysis of PFGE patterns generated at three different laboratories in study phase II correctly identified 3 isolates from each of 10 outbreaks among the isolates investigated.

    Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

    Techniques: Generated